Expression of cathepsin-B and -D in rat's brain after traumatic brain injury
Zhang, Y.-b.; Chen, X.-p.; Tao, L.-y.; Qin, Z.-h.; Li, S.-x.; Yang, L.; Yang, J.; Zhang, Y.-g.; Liu, R.
Fa Yi Xue Za Zhi 22(6): 404-406; 410
2006
ISSN/ISBN: 1004-5619 PMID: 17285857 Document Number: 595044
To study the expression of cathepsin-B and -D in different time point after traumatic brain injury. Traumatic brain injury (TBI) model was established on rats, cathepsin-B and cathepsin-D immunofluorescence staining and confocal microscope analysis were performed. Positive cells were counted by confocal microscope and image analysis techniques were used to determine the morphological changes in each group. Immunofluorescence staining results showed that cathepsin-B was activated 1 hour after TBI while cathepsin-D was not activated until 12hour after TBI. Both of them got to their peak during 4 to 8days, and kept a high level of activating 32days after TBI. Cathepsin-B and -D positive cells did not merge with caspase-3 positive cells until 6 h after TBI. Cathepsin-B and -D could be the diagnostic markers of TBI and can estimating time course of lateral TBI. They blocked caspase-3 activation at the beginning period after TBI and started to promote cell death with caspase-3 6 h after TBI.