Chemical diagnosis of carbamazepine intoxication and its usefulness in toxicological jurisprudence
Wachowiak, R.; Tobolski, J.ła.; Klimaszyk, D.
Archiwum Medycyny Sadowej i Kryminologii 55(1): 94-100
2005
ISSN/ISBN: 0324-8267 PMID: 15984129 Document Number: 594416
Intoxication with carbamazepine (Amizepine, Tegretol) represents a significant problem of clinical and forensic toxicology which frequently develops independently of the commonly used monitored therapy. In the study a technique of qualitative and quantitative analysis applied to carbamazepine (CBZ) as well as its metabolites carbamazepine 10, 11-epoxide (CBZ-E) and 10, 11-dihydroxycarbamazepine (CBZ-DH) in biological material using high-performance liquid chromatography (HPLC) was presented. The studies also involve isolation of studied compounds using solid phase extraction, SPE-Abselut NEXUS (Varian), establishment of calibration range and validation of analytical parameters. This was followed by a discussion of results obtained in routine diagnostic investigations in cases of intoxications, corroborating practical suitability of the designed technique.