Methicillin-resistant Staphylococcus aureus strains in Buenos Aires teaching hospitals: replacement of the multidrug resistant South American clone by another susceptible to rifampin, minocycline and trimethoprim-sulfamethoxazole
Gardella, N.; Picasso, R.; Predari, S.C.; Lasala, M.; Foccoli, M.; Benchetrit, G.; Famiglietti, A.; Catalano, M.; Mollerach, M.; Gutkind, G.
Revista Argentina de Microbiologia 37(3): 156-160
2005
ISSN/ISBN: 0325-7541 PMID: 16323667 Document Number: 594338
The aim of this study was to characterize methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered from different infectious sites of hospitalized patients at two university hospitals. Fourteen isolates were analyzed by repetitive sequence based PCR (Rep-PCR), randomly amplified polymorphic DNA assay (RAPD-PCR), and pulsed-field gel electrophoresis (PFGE). We found that a prevalent clone of MRSA, susceptible to rifampin, minocycline, and trimethoprim/sulfamethoxazole (RIFS, MINS, TMSS) was present in both hospitals in replacement of the multiresistant MRSA South American clone, previously described in these hospitals. The staphylococcal chromosomal cassette (SCCmec) type I element was detected in this new clone.