Top Abstract Purchase Related
Home
>
Node
>
Subnode

Application with single-stranded probes in randomized terminal linker-dependent PCR

Rao, C.-l.; Wu, Q.; Heng, Z.-c.

Wei Sheng Yan Jiu 34(2): 172-174

2005

ISSN/ISBN: 1000-8020
PMID: 15952655
Document Number: 592736
Preparing single-stranded (ss) probes in place of double-stranded (ds) probes to improve the hybridization efficiency and specificity of randomized terminal linker-dependent PCR (RDPCR). Using asymmetric PCR and single-primer PCR to prepare ss probes of extron 7 of p53 gene in rat, then comparing the results hybridized with ss probes and ds probes. Preparation of ss probes by asymmetric PCR and single-primer PCR gets success. Hybridization results showed that the ss probes could get better signals and less noise than ds probes. In comparison with ds probes, the application of ss probes can increase the hybridization sensitivity and specificity of RDPCR.

Document emailed within 1 workday
Secure & encrypted payments

Silk, G.W.; Wu, M. 1993: 3' transcript mapping of the Chlamydomonas reinhardtii chloroplast psbK mRNA using a novel method to prepare 3' end-labeled single-stranded DNA probes Biotechniques 14(5): 830-833
Epstein, W.V. 1975: Specificity of SLE serum antibody for single-stranded and double-stranded DNA configuration Journal of Rheumatology 2(2): 215-220
Timofeev, V.P.; Arutiunian, A.I.; Petrov, A.I. 1990: Segmental flexibility of single-, double-, and triple-stranded polyribonucleotides from the data of spin label method. Formation of the triple-stranded poly(A.A.U) polynucleotide helix Molekuliarnaia Biologiia 24(1): 140-155
Sukprasert, M.; Rattanasiri, S.; Xu, K. 2013: The comparison of DNA quantity between full and half volume single cell whole genome amplification by linker-adapter PCR technique Journal of the Medical Association of Thailand 96(11): 1491-1497
On, S.C.J.; Linkner, R.V.; Haddican, M.; Yaroshinsky, A.; Gagliotti, M.; Singer, G.; Goldenberg, G. 2014: A single-blinded randomized controlled study to assess the efficacy of twice daily application of sinecatechins 15% ointment when used sequentially with cryotherapy in the treatment of external genital warts Journal of Drugs in Dermatology: Jdd 13(11): 1400-1405
Strel'tsov, S.A.; Lysov, I.P.; Semenov, T.E.; Vengerov, I.I.; Khorlin, A.A.; Surovaia, A.N.; Gurskiĭ, G.V. 1991: Interaction of trivaline with single-stranded polyribonucleotides Molekuliarnaia Biologiia 25(4): 1040-1060
Rae, B.P.; Hillman, B.I.; Tartaglia, J.; Nuss, D.L. 1989: Characterization of double-stranded RNA genetic elements associated with biological control of chestnut blight: organization of terminal domains and identification of gene products EMBO Journal 8(3): 657-663
Shabarova, Z.A. 1991: Design and applications of single and double stranded DNA analogs Nucleic Acids Symposium Series 24: 99-102
Medori, R.; Tritschler, H.J.; Gambetti, P. 1992: Production of single-stranded DNA for sequencing: an alternative approach Biotechniques 12(3): 346-350
Kaltenboeck, B.; Spatafora, J.W.; Zhang, X.; Kousoulas, K.G.; Blackwell, M.; Storz, J. 1992: Efficient production of single-stranded DNA as long as 2 kb for sequencing of PCR-amplified DNA Biotechniques 12(2): 164; 166 168-171
Oberosler, P.; Hloch, P.; Ramsperger, U.; Stahl, H. 1993: p53-catalyzed annealing of complementary single-stranded nucleic acids EMBO Journal 12(6): 2389-2396
Valeev, A.K. 1980: Calculation of sedimentation curves for single-stranded DNA with induced breaks Molekuliarnaia Biologiia 14(6): 1234-1242
Valentine, J.E.; Boyle, M.J.; Sewell, W.A.; Gough, N.M. 1992: Presence of single-stranded DNA in PCR products of slow electrophoretic mobility Biotechniques 13(2): 222-224
Dean, M.; Gerrard, B. 1991: Helpful hints for the detection of single-stranded conformation polymorphisms Biotechniques 10(3): 332-333
Yang, Y.L.; Reis, L.F.; Pavlovic, J.; Aguzzi, A.; Schäfer, R.; Kumar, A.; Williams, B.R.; Aguet, M.; Weissmann, C. 1995: Deficient signaling in mice devoid of double-stranded RNA-dependent protein kinase EMBO Journal 14(24): 6095-6106
Bugreeva, I.P.; Bugreev, D.V.; Nevinskiĭ, G.A. 2007: Interaction of single-stranded DNA with the second DNA-binding site of RecA nucleoprotein filament Molekuliarnaia Biologiia 41(3): 524-534
Eilat, D.; Steinberg, A.D.; Schechter, A.N. 1978: The reaction of SLE antibodies with native, single stranded RNA: radioassay and binding specificities Journal of Immunology 120(2): 550-557
Pirtskhalava, M.V.; Chernen'kaia, S.N.; Lukerenko, R.V.; Vasil'ev, A.N. 1990: Single- and double-stranded DNA from mouse liver after exposure to carbon tetrachloride Ukrainskii Biokhimicheskii Zhurnal 62(5): 92-95
Veĭko, N.N.; Gromova, E.S.; Shabarova, Z.A. 1979: Interaction of gene 5 protein of phage f1 with single and double-stranded DNA and polynucleotides Molekuliarnaia Biologiia 13(5): 1136-1146
Carsana, A.; Lavitrano, M.L.; Libonati, M. 1984: Proteins with preferential affinity for single-stranded DNA in tissues of Octopus vulgaris lam Italian Journal of Biochemistry 33(2): 88-97