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Effects of berberine on adiponectin mRNA expression in 3T3-L1 adipocyte

Gu, W.; Zeng, W-heng.; Hu, H-ying.

Zhongguo Zhong Yao Za Zhi 30(4): 286-288

2005

ISSN/ISBN: 1001-5302
PMID: 15724409
Document Number: 592603
Objective: To explore the effects of berberine and insulin on adiponectin mRNA expression in 3T3-L1 adipocyte. Method: The 3T3-L1 adipocyte was treated with berberine and insulin for 48 hours, the level of adiponectin mRNA in 3T3-L1 adipocyte expression was determined with semiquantity RT-PCR using beta-actin as internal reference. Result: The level of adiponetin mRNA in 3T3-L1 adipocyte was increased after treated with berberine only (P < 0.05), the effect of berberine was inhibited by high concentration insulin(P < 0.05). Conclusion: in vitro I berberine increases the expression of adiponectin in 3T3-L1 adipocyte, insulin inhibits the effect of berberine.

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