Fabrication of decellularized scaffold of homograft bioprosthetic tube valved with two kinds of cell detergents
Feng, B.; Lui, Y.; Xie, N.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 19(4): 310-313
2005
ISSN/ISBN: 1002-1892 PMID: 15921326 Document Number: 590917
To compare the effect of fabricating decellularized scaffold of homograft bioprosthetic tube valved (HBTV) with two kinds of cell detergents and to provide a homograft bioprosthetic scaffold for fabrication of tissue-engineering heart valve (TEHV). The active cells in the HBTV, which conserved by liquid nitrogen, were decellularized by low osmotic pressure of Tris buffer, in which containing sodium dodecylsulphate (SDS) and deoxycholic acid (DOA) respectively. The leaflets or aortic wall was fixed with fixative and stained with hematoxylin and eosin, collagen fibers or elastic fibers for observation and photographs by light microscope or by scanning electron microscope (SEM) after decellularized. When the leaflets of HBTV were incubated together with 0.03% SDS or 0.5% DOA of Tris buffer respectively for 48 hours, the active endothelial cells (ECs) in the leaflets were not only decellularized completely, but also reserved the collagen fibers or elastic fibers integrally, which is two of the main components of extracellular matrix (ECM). A part of fibroblast in the center leaflets was reserved. The morphologic structure of leaflets after decellularized was not significantly different from that before decellularized. The concentration of SDS was increased to 0.1% when decellularized the cells of aortic wall, but DOA was still kept 0.5%. The better decellularized scaffold of HBTV obtained was disposed by 0.03%-0.1% SDS or 0.5% DOA, which was advantageous to adhesiveness and amplification of implantation cells on the decellularized scaffold of HBTV in order that HBV re-endothelialized or for the TEHV fabricated in vitro.