Effects of conjugated linoleic acid on the expression of critical enzymes of linoleic acid metabolism in tumor cell
Zhang, J-shu.; Chen, B-qing.; Gao, Y-hui.; Yang, Y-mei.; Wang, X-lin.; Song, Y.; Sun, W-guang.
Zhonghua Yu Fang Yi Xue Za Zhi 39(3): 195-198
2005
ISSN/ISBN: 0253-9624 PMID: 15938855 Document Number: 590731
Objectives To study the effects of c9, t11-conjugated linoleic acid (c9, t11-CLA) on critical enzymes of linoleic acid metabolism in stomach granular cell(SGC-7901). Methods SGC-7901 was treated with c9,t11-CLA by 200, 100,50 or 25 mu mol/L for 24 hours. The effects of c9,t11-CLA on the cell proliferation was measured by monotetrazolium and the expression of Delta 6-desaturase, Delta 5-desaturase, COX-1, COX-2,5-LOX mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR). Results At a concentration of 200,100,50, or 25 mu mol/L, c9, t11-CLA suppressed the proliferation of SGC-7901 by 54. 3 % 20. 5 % 10. 5 % and 2. 93 %. The c9, t11-CLA might decrease the expression of COX-2 mRNA, and increase the expression of Delta 6-desaturase and COX-1 in SGC-7901, but might not affect Delta 5-desaturase and 5-LOX. Conclusion The effects of c9,t1-CLA on the COX and Delta 6-desaturase might play an important role in mediating the ability of c9,t11-CLA as to inhibiting the proliferation of tumor cells, and the anticancer activity by c9,t11-CLA might be associated with the linoleic acid metabolism.