Determination of chloramphenicol residue in fish and shrimp tissues by gas chromatography with a microcell electron capture detector
Ding, S.; Shen, J.; Zhang, S.; Jiang, H.; Sun, Z.
Journal - Association of Official Analytical Chemists 88(1): 57-60
2005
ISSN/ISBN: 0004-5756 PMID: 15759726 Document Number: 589226
A gas chromatography method with microcell electron capture detection was developed for the determination of chloramphenicol residue in fish and shrimp muscle tissues. The tissue samples were extracted with ethyl acetate, defatted with hexane, and derivatized with Sylon BFT [N, O-bis (trimethylsilyl) trifluoroacetamide-trimethylchlorosilane (99 + 1)]. The limit of detection was 0.04 ng/g and the limit of quantitation 0.1 ng/g. Average recoveries were 70.8-90.8% for fish and 69.9-86.3% for shrimp, respectively. The method was validated for the determination of practical samples.