Feasibility of muscle-derived cell autotransplantation as a treatment for post-prostatectomy urinary incontinence
Yao, Q.-s.; Ye, Z.-q.; Chen, C.-b.; Wang, X.-k.; Wang, W.-m.; Chen, L.-x.
Zhonghua Nan Ke Xue 11(4): 272-274; 277
2005
ISSN/ISBN: 1009-3591 PMID: 15921257 Document Number: 589071
To explore the feasibility of muscle-derived cell autotransplantation in the treatment of post-prostatectomy urinary incontinence. Skeletal muscle-derived cells (MDC) were isolated and purified by replate technique from 6 female SD rats, and then transduced with adenovirus carrying Lac-Z gene. About 5 x 10(6) of the transduced cells were injected autologously into the bladder neck of the animals. Tissues were harvested after 5 and 15 days for histological examination and X-gal staining. At 5 and 15 days after the autologous MDC transplantation, histological examination revealed no apparent sign of inflammation and inflammatory cell invasion, and X-gal staining showed a large number of cells dyed blue, indicating the survival of the autologous cells. Autotransplanted MDCs can survive permanently. Autologous muscle stem cell injection can be an effective treatment for post-prostatectomy urinary incontinence.