Development and potential of a biomimetic chitosan/type II collagen scaffold for cartilage tissue engineering
Shi, D-hai.; Cai, D-zhang.; Zhou, C-ren.; Rong, L-min.; Wang, K.; Xu, Y-chun.
Chinese Medical Journal 118(17): 1436-1443
2005
ISSN/ISBN: 0366-6999 PMID: 16157047 Document Number: 588831
Background Damaged articular cartilage has very limited capacity for spontaneous healing. Tissue engineering provides a new hope for functional cartilage repair. Creation of an appropriate cell carrier is one of the critical steps for successful tissue engineering. With the supposition that a biomimetic construct might promise to generate better effects, we developed a novel composite scaffold and investigated its potential for cartilage tissue engineering.Methods Chitosan of 88% deacetylation was prepared via a modified base reaction procedure. A freeze-drying process was employed to fabricate a three-dimensional composite scaffold consisting of chitosan and type 11 collagen. The scaffold was treated with I-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide. Ultrastructure and tensile strength of the matrix were carried out to assess its physicochemical properties. After subcutaneous implantation in rabbits, its in vivo biocompatibility and degradability of the scaffold were determined. Its capacity to sustain chondrocyte growth and biosynthesis was evaluated through cell-scaffold co-culture in vitro.Results The fabricated composite matrix was porous and sponge-like with interconnected pores measuring from 100-250 mu m in diameter. After cross-linking, the scaffold displayed enhanced tensile strength. Subcutaneous implantation results indicated the composite matrix was biocompatible and biodegradable. In intro cell-scaffold culture showed the scaffold sustained chondrocyte proliferation and differentiation, and maintained the spheric chondrocytic phenotype. As indicated by immunohistochemical staining, the chondrocytes synthesized type H collagen.Conclusions Chitosan and type II collagen can be well blended and developed into a porous 3-D biomimetic matrix. Results of physico-chemical and biological tests suggest the composite matrix satisfies the constraints specified for a tissue -engineered construct and may be used as a chondrocyte carrier for cartilage tissue engineering.