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Studies on transplanting suspension-cultured protocorms of Dendrobium candidum onto solid culture medium

Hou, P-yong.; Guo, S-xing.

Zhongguo Zhong Yao Za Zhi 30(10): 729-732

2005

ISSN/ISBN: 1001-5302
PMID: 16075706
Document Number: 588377
Objective: The suspension-cultured protocorms of Dendrobium candidum were transplanted on the solid culture medium for studying the factors influencing their differentiation and growth. Method: The growth and differentiation of protocorms were detected when different base-media, plant phytohormones, carbohydrates and pH were applied. Result and Conclusion: The diluted MS medium promotes the growth of protocorms but inhibite differentiation. The phytohormone NAA, IAA, IBA and GA are helpful to the growth and differentiation of protocorms, however, the low pH values and presence of autoclaved fungi have suppressive effects on protocorms. The fungi also induce morphological variation of protocorms.

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