Production of recombinant fragment of VacA protein and development of non-invasive method for the diagnosis of Helicobacter pylori infection
Belyĭ, I.F.; Sheklakova, L.A.; Vakhrameeva, M.S.; Zhukhovitskiĭ, V.G.; Petrukhin, V.G.; Belaia, I.A.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 2005(1): 32-35
2005
ISSN/ISBN: 0208-0613 PMID: 15790031 Document Number: 588262
Vacuolizing toxin (VacA) Helicobacter pylori is an important factor of pathogenicity of Helicobacter pylori and a basic marker in tile diagnosis of helicobacteriosis and related diseases. A coagulation-based diagnostic test-system was elaborated for the detection of VacA in clinical samples. A fragment of vacA was cloned, for the Purpose, in Escherichia coli and expressed in preparative quantities; the coded protein was purified and used in raising the diagnostic serum. Tile thus designed coagulating test-system was successfully tested under the modeling conditions with clinical samples. Therefore, the designed express method can be used for the invasion-free determination of VacA in patients with gastric and duodenal pathologies.