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A study of the peripheral quaternary ligand binding site of cholinesterase with the use of ethidium bromide

Vetkin, D.O.; Vlaskin, D.N.; Gaĭnulina, E.T.; Ryzhikov, S.B.; Taranchenko, V.F.

Biofizika 50(5): 793-796

2005

ISSN/ISBN: 0006-3029
PMID: 16248152
Document Number: 588028
The peripheral binding site of horse serum cholinesterase (EC 3.1.1.7) for quaternary ligands was investigated by fluorescent probing with the use of ethidium bromide. Spectral evidence for the participation of the tryptophan indole group of the peripheral site of horse serum cholinesterase in the formation of a cholinesterase complex with ethidium bromide is presented. The mechanism of cholinesterase effect on ethidium bromide fluorescence is proposed.

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