The expression and significance of myocardial cathepsin L in dilated cardiomyopathy
Yu, X.-h.; Zhang, X.-g.; Li, S.-j.; Wang, S.-j.; Zhao, G.; Chen, R.-z.; Yang, Y.-z.
Zhonghua Nei Ke Za Zhi 44(7): 495-498
2005
ISSN/ISBN: 0578-1426 PMID: 16080836 Document Number: 584651
To clarify the expression of myocardial cathepsin L and its significance in the pathogenesis of dilated cardiomyopathy (DCM). Myocardial tissue specimens derived from 20 patients undergoing orthotopic cardiac transplantation and 5 normal myocardium samples collected at autopsy from sudden death as controls were studied. The expression of cathepsin L was detected with immunohistochemistry, real time PCR and Western Blotting, respectively. At the same time, the relationship between the Cathepsin L mRNA expressional levels and the myocardial function (ejection fraction, EF) was investigated. The expression levels of cathepsin L mRNA and protein in DCM group were markedly elevated compared with those in control group and the expression levels of cathepsin L mRNA had significantly negative correlation with the myocardial function (ejection fraction, EF). Cathepsin L is markedly elevated in DCM myocardial tissue and it may participate in the pathogenesis of DCM.