Molecular biological identification of Batai virus isolated in China
Fu, S.-h.; Sun, X.-h.; Wang, H.-y.; Cao, Y.-x.; Wang, H.-q.; Liu, W.-b.; Tao, S.-j.; Liang, G.-d.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 19(4): 331-334
2005
ISSN/ISBN: 1003-9279 PMID: 16415989 Document Number: 584439
To study the molecular characteristics of YN92-4 strain isolated from mosquitoes in Yunnan Province and define its classification. The S segment of YN92-4 strain was amplified and sequenced by 2 different sets of primers. The phylogenic tree of S fragment was constructed by Phylip bio-software. The amino acid sequences of N and NSs proteins were also studied. YN92-4 strain could be amplified by 2 sets of primers respectively, S segment showed a highest homology with Batai virus (X73464), reached 96.4%, the homology of protein N and NSs amio-acid sequence with Batai virus was 99.1% and 98% respectively. The YN92-4 strain belongs to Batai virus, this is the first report of molecular biological identification of Batai virus in China.