Reconstruction of dentin-pulp complex structure by tissue engineering technology
Guo, H.-y.; Wu, B.-l.; Guo, X.-m.; Yang, C.; Xu, P.; Wang, C.-y.
Zhonghua Kou Qiang Yi Xue Za Zhi 40(6): 511-514
2005
ISSN/ISBN: 1002-0098 PMID: 16329844 Document Number: 584400
To investigate the possibility of reconstruction of dentin-pulp complex by tissue engineering technology. Rat dental pulp stem cells were seeded into HA-TCP scaffold and incubated for 20 hours in vitro. Then the cell-scaffold complex was implanted subcutaneously into the dorsal side of nude mice. 8 weeks postimplantation, the samples were extracted for histological and immunohistochemical examinations. Three strata of tissue were observed in the hole of HA-TCP scaffold. They were dentin-like tissue, predentin-like tissue and pulp-like tissue respectively from the inner surface of the pore to the center. Dentin tubules were obvious in predentin-like and dentin-like tissue lining from the pulp-like tissue through predentin-like tissue and dentin-like tissue. Cells localized along the edge of pulp-like tissue were dense and polarized, resembling odontoblasts. Immunohistochemical study demonstrated DSP and DMP1 expression in these odontoblast-like cells and in the area of predentin-like tissue. Tissue-engineered rat dentin-pulp complex was reconstructed by seeding HA-TCP scaffold with rat dental pulp stem cells.