Effects of millimeter wave on gap junctional intercellular communication in human keratinocytes
Chen, Q.; Zeng, Q-li.; Lu, D-qiang.; Jiang, H.
Zhonghua Yu Fang Yi Xue Za Zhi 38(1): 8-10
2004
ISSN/ISBN: 0253-9624 PMID: 14989890 Document Number: 584364
Objective To explore the effect of millimeter wave (MW) at low power density on gap junctional intercellular communication ( GJIC) in human keratinocytes ( HaCaTs). Methods Fluorescence recovery after photobleaching (FRAP) technique was employed to determine effect of 30.16 GHz MW exposure at 1.0 and 3.5 mW / cm2 on GJIC with laser confocal scanning microscope. Results FRAP analysis revealed that 12-O-tetradecanoylphorbol-13-acetate (TPA) at a dose of 5 mug/L could inhibit GJIC in HaCaTs. Fluorescence recovery rate fell from (55 +/- 17) % in the controls to (34 +/- 13) % after photobleaching, with a very significant difference (P 0.001). Exposure to MW alone for one hour at either 1.0 mW/cm2 or 3.5 mW/cm2 did not affect GJIC, with fluorescence recovery rates of (52 +/- 16) % and (50 +/- 17) %, respectively. GJIC suppression induced by TPA was weakened by MW combined with 5 mug/L TPA treatment for one hour, which could be partially recovered by exposure to 1.0 mW/cm2 MW with fluorescence recovery rate of (47 +/- 16) %, P 0.01, and fully recovered by exposure to 3.5 mW/cm2 MW with fluorescence recovery rate of (50 +/- 16) %, P 0.001, with a very significant difference. Conclusions GJIC suppression induced by TPA could be eliminated or diminished by exposure to millimeter wave in HaCaTs.