Determination of tyrosine- and tryptophan-containing peptides in plasma by absorption in UV spectral region
Gavrilov, V.B.; Lobko, N.F.; Konev, S.V.
Klinicheskaia Laboratornaia Diagnostika 3: 12-16
2004
ISSN/ISBN: 0869-2084 PMID: 15137197 Document Number: 583155
A method for determining the cumulative concentration of tyrosine- and tryptophan-containing peptides (TSP and TPP) in blood plasma: 0.2 ml of blood plasma is added 0.1 ml of 1.8 M chloric acid, the sample is centrifuged, the supernatant (0.2 ml) is added 1.8 ml of 0.7 M NaOH and the optic density is measured at 290 nm in the quarts or plastic flask. Equations were derived to calculate separately the concentrations of TSP and TPP, whose normal values are 1.28 +/- 0.31 and 0.25 +/- 0.06 mmol/l, respectively. The increasing total content of peptides in thyrotoxicosis was shown to be mainly conditioned by a higher TSP concentration (a 2.4-fold increase). Therefore, TSP is a more sensitive marker in case of endogenous intoxication versus the generally known index of the cumulative content of oligopeptides.