Studies on the effects of lead on the growth and differentiation of hippocampal neural cells as well as the expression of Oct-2
Chen, J.; Zhu, W-guo.; Chen, Q-sheng.; Lu, L.; Chen, X-min.
Zhonghua Yu Fang Yi Xue Za Zhi 38(3): 151-154
2004
ISSN/ISBN: 0253-9624 PMID: 15182478 Document Number: 582760
Objective In order to explore the effects of lead on the growth and development of cultured hippocampal neural cells and on the expression of Oct-2, the II subtype POU domain protein. Methods Experiment cell model was established using primary culture of hippocampal neural cells from SD rat embryos. Target cells were exposed to lead acetate in the different concentrations, i. e. 10-1, 100,101, 102,103 mumol/L, while the control group was given the same quantity of the culture medium. The mmunohistochemistry method was utilized to detect the expressions of Neurofilament (NF) and Glial Fibrillary Acidic Protein (GFAP), the markers for neuron and astrocyte, repectively, and the expression of Oct-2 as well. Results The results showed that 10 mumol/L lead acetate treatment caused diminishing of neuronal cell body and the decreases of both axon lengths and inter-cellular connections. In addition, 1 mumol/L lead acetate significantly increased the number of GFAP-positive cells compared with the control group (P 0.05). By image analysis system, 1 mumol/L lead acetate treatment was found to induce a statistically significant increase of the positive area rate concerning Oct-2 expression in hippocampal neurons and astrocytes, while both positive area rate and integral density of light of Oct-2 expression were found to increase markedly. in the groups treated by 10 mumol/L lead acetate(P 0.01). Conclusions Lead acetate treatment may contribute to the inhibitions of both growth and differentiation of hippocampus neurons, and to the stimulation of glialcell hyperplasia simultaneously. In addition, the CNS impairments caused by lead is partly correlated with the enhancement of Oct-2 expression.