Experimental study on basic fibroblast growth factor combined slow-releasing degradable membrane to prevent tendon adhesion
Sha, D-feng.; Xin, C-tai.; Yang, X-xia.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 18(2): 148-151
2004
ISSN/ISBN: 1002-1892 PMID: 15065419 Document Number: 581834
To study the adhesion-preventing effect of basic fibroblast growth factor (bFGF) combined slow-releasing degradable membrane. The bFGF combined slow-releasing degradable membrane was made from bFGF and the reagent which could promote fibrinogen synthesize. Sixty-six SD rats were divided into groups A, B, C randomly (22 rats each group). In group A, sutured achilles tendon were encapsulated with bFGF combined slow-releasing degradable membrane; in group B, sutured achilles tendon were encapsulated with degradable membrane without any drug; in group C, achilles tendon were only sutured. Ninety days later, light-microscope, electron-microscopoe, figure-analysing, hydroxyproline content, extent of peritendon adhesion and biomechanic test were evaluated. 1. The amount of fibroblast and fibrinogen inside the sutured tendon in group A was larger than that in its peripheral connective tissue and in groups B and C (P < 0.05). The content of hydroxyproline and the ultimate tensile strength in group A was higher than those in groups B and C (P < 0.01). 2. The peripheral tissue in group A almost remains the formal loose connective tissue, but it became dense connective tissue in groups B and C and grew into the tendon. Moreover, the extent of adhesion in group A was lesser than that in groups B, C according to the mensuration of peritendon adhesion. The bFGF combined slow-releasing degradable membrane can make the intrinsic healing of tendon faster than peripheral connective tissue proliferation, so it can prevent the formation of adhesion.