Effects of fibroblast growth factor 8, fibroblast growth factor 9 and keratinocyte growth factor on multiplication and locomotion in human teratocarcinoma cells in vitro
Granerus, M.; Engström, W.
Anticancer Research 23(2b): 1313-1316
2003
ISSN/ISBN: 0250-7005 PMID: 12820388 Document Number: 560289
Background: The effects of three members of the heparin binding growth factor family, FGF-8, FGF-9 and KGF, on the human embryonal carcinoma-derived cells line Tera 2 were examined. Materials and Methods: Cell numbers as well as cell migration was examined at clonal level by microscopy. Results: It was found that each of these factors promoted Tera 2 cell multiplication, albeit with different efficacy. Whereas a dramatic effect on cell numbers was observed after the addition of 1-10 mug FGF-9/ml, a lesser effect could be achieved by FGF-8 or KGF. In contrast, KGF expressed the most potent effect on cell locomotion at a higher concentration (100 mug/ml). Even though high concentrations of FGF-8 and FGF-9 did stimulate cell locomotion, this effect was substantially smaller than that of KGF. Conclusion: All three FGFs exert a dual effect on Tera 2 cell survival and locomotion.