Effect of activation regimens for rat oocytes on full-term development after round spermatid injection
Kato, M.; Ishikawa, A.; Hochi, S.; Hirabayashi, M.
Contemporary Topics in Laboratory Animal Science 43(2): 13-15
2004
ISSN/ISBN: 1060-0558 PMID: 15053501 Document Number: 578233
The present study was designed to compare the effect of two activation regimens for rat oocytes on full-term development after round spermatid injection (ROSI). Oocytes were treated either with 1.25 mM SrCl2 for 20 min or with two direct-current (DC) pulses at 100 V/mm for 99 micro sec followed by exposure to 2 mM 6-dimethylaminopurine (6-DMAP) for 20 min. Single spermatids then were injected into the oocytes that were ready for polar body extrusion. The proportions of oocytes surviving injection and exhibiting pronuclear formation were comparable between the SrCl2 group (76.8% and 45.6%, respectively) and the DC+6-DMAP group (78.3% and 34.9%, respectively). More SrCl2-activated ROSI oocytes (44.2%) cleaved into the two-cell stage than did those activated with DC+6-DMAP (34.7%). However, the proportions of the two-cell embryos developing to morulae/blastocysts and live offspring were significantly (P=0.0005 and 0.042) lower in the SrCl2 group (12.3 and 6.1%, respectively) than the DC+6-DMAP group (36.1 and 14.6%, respectively). Our results indicate DC+6-DMAP is better than SrCl2 for oocyte activation prior to successful ROSI of rats.