Evaluation of cisplatin-DNA crosslinks formation with UV-C application by the alkaline Comet-assay
Hovhannisyan, G.G.; Haroutunyan, T.S.; Arutyunyan, R.M.
Experimental Oncology 26(3): 240-242
2004
ISSN/ISBN: 1812-9269 PMID: 15494695 Document Number: 575152
The aim of the present study was to investigate DNA damage induced by widely applied anticancer preparation cisplatin (cis-DDP) at doses comparable with therapeutic ones in leukocytes of healthy donors. Methods: DNA damage and repair was estimated by the single cell gel electrophoresis or Comet-assay. For estimation of cis-DDP-induced DNA crosslinks the combined treatment by cis-DDP with the DNA damaging agent UV-C was used. The results obtained indicate that cis-DDP is forming crosslinks with DNA in human leukocytes and significantly reduce UV-C-induced DNA migration. Conclusion: The Comet-assay with UV-C application is a useful tool to detect cis-DDP-induced DNA crosslinks. Data on the cis-DDP-induced DNA damage in vitro may be important for their extrapolation on in vivo level.