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Adaptation of three Babesia divergens isolates to continuous culture in rat erythrocytes

Musa, N.B.; Abdel Gawad, M.A.

Journal of the Egyptian Society of Parasitology 34(1): 333-344

2004

ISSN/ISBN: 1110-0583
PMID: 15125537
Document Number: 575127
Three Babesia divergens isolates have been cultured continuously for 6 months in rat erythrocytes using the candle jar technique. One isolate was already rat-adapted, the other two became adapted to rats through continuous culturing in rat erythrocytes. Babesia was cultured in rat erythrocytes in RPMI medium supplemented with 20% foetal calf serum. The highest parasitaemia was 35% and multi-parasitization of red blood cells was often observed. Cultures of B. divergens remained infective to splenectomized rats. Cultures with high parasitaemias contained a large number of extra-cellular merozoites, when separated from the red blood cells, they retained their infectivity.

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