Effects of genistein on NOS, GSH-Px activities and NO, GSH, MDA contents in MCF human breast cancer cells
Sun, S.'a.; Zhao, X.; Zhao, L.
Wei Sheng Yan Jiu 33(4): 468-469
2004
ISSN/ISBN: 1000-8020 PMID: 15461279 Document Number: 572038
To investigate the mechanism that the genistein inhibit the MCF human breast cancer cell. The activities of glutathione peroxidase (GSH-Px), nitric oxide synthetase (NOS) and the contents of nitric oxide (NO), glutathione (GSH), malondialdehyde (MDA) both in culture supernatant and cells were examined by enzyme method and spectrophotometry respectively. With genistein increasing, the activities of GSH-Px, NOS and the contents of NO increased significantly (P < 0.05), while the contents of GSH, MDA decreased both in culture supernatant and cells (P < 0.05). Genistein can affect NO synthesis and antioxidation in MCF breast cancer cells. It may be one of the mechanisms that the genistein inhibit the MCF breast cancer cells growth.