Genistein exerts multiple suppressive effects on human breast carcinoma cells
Shao, Z.; Wu, J.; Shen, Z.
Zhonghua Zhong Liu Za Zhi 22(5): 362-365
2000
ISSN/ISBN: 0253-3766 PMID: 11778268 Document Number: 515034
To study the suppressive effects of genistein on progression of breast carcinoma. In vitro cell culture, animal studies, zymography, Northern and Western blot were used in this study. Genistein inhibited in vitro invasion of MCF-7 and MDA-MB-231 cells. This inhibition was associated with down-regulation of MMP-9 and up-regulation of TIMP-1. These effects were also demonstrated in vivo. Genistein inhibited growth of the breast cancer cells transplanted in nude mice. The growth inhibitory effect was associated with increased tumor cell apoptosis, and up-regulated p21WAF1/CIP1 expression. Furthermore, genistein inhibited angiogenesis as shown by decreased vessel density and decreased production and release of VEGF and TGF-beta 1. Genistein exerts multiple suppressive effects on breast carcinoma. It may help halt its progression.