Biotransformation of dehydroepiandrosterone by hairy root cultures of Anisodus tanguticus
Liu, Y.; Cheng, K-di.; Zhu, P.; Feng, W-hua.; Meng, C.; Zhu, H-xin.; He, H-xia.; Ma, X-jun.
Yao Xue Xue Bao 39(6): 445-448
2004
ISSN/ISBN: 0513-4870 PMID: 15491103 Document Number: 570308
The structure of dehydroepiandrosterone (DHEA) was modified. Hairy root cultures of A. tanguticus were used to perform biotransformation of DHEA, and chromatographic and spectral techniques were used to isolate and identify the products. (1) The MS medium without plant hormone was suitable for the growth of the hairy root. (2) DHEA was converted into five products: androst-4-ene-3,17-dione (I); 6 alpha -hydroxyandrost-4-ene-3,17-dione (II); 6 alpha ,17 beta -dihydroxyandrost-4-ene-3-one (III); androst-4-ene-3,6,17-trione (IV) and 17 beta -hydroxyandrost-4-ene-3-one (V). This is the first use of hairy root cultures of A. tanguticus for the biotransformation of DHEA and five DHEA-related compounds were obtained.