Effects of shouwu-huanjing recipe on human sperm motility and fertility in vitro
Zeng, J.-X.; Dai, X.-H.; Liu, J.-H.; Yang, J.-H.; Chen, J.-F.; Lin, M.-N.
Zhonghua Nan Ke Xue 9(6): 476-479
2003
ISSN/ISBN: 1009-3591 PMID: 14574818 Document Number: 565484
To observe the effect of Shouwu-Huanjing Recipe(SWHJR) medicated serum on human sperm motility and fertility in vitro. Human sperm was co-cultured with SWHJR medicated serum in vitro. Human sperm motility was evaluated by computer-assisted semen analysis(CASA). The acrosome reaction and the capability of penetrating zona-free hamster eggs were also observed. The co-cultured SWHJR medicated serum significantly increased the sperm motion velocity(VAP, VCL, VSL) (P < 0.01), the amplitude of lateral head movement (ALH) and the beat frequency of flagellum(BCF), the density of progressive motility sperms (P < 0.05), the acrosome reaction rate(P < 0.001), the fertilization rate(FR) and the fertilization index (FI) in sperm penetration assay(SPA) test (P < 0.01). The stimulation of SWHJR medicated serum occurred in dose-dependent manner. SWHJR can improve human sperm motility and fertility.