Detection technique of microsatellites polymorphism and its application in conservation genetics
Yan, P.; Wu, X.; Shi, Y.; Zhang, F.
Ying Yong Sheng Tai Xue Bao 14(3): 461-464
2003
ISSN/ISBN: 1001-9332 PMID: 12836562 Document Number: 564264
Microsatellites are a novel molecular genetic marker, and offer unusual advantages, e.g., with the using of a very few sample, they can be amplified by PCR, which are particularly appropriate in the research of conservation genetics. In this paper, the structure of microsatellites and the principle of the detection technique of microsatellites polymorphism were illustrated, and the advantages and the application of this detection technique were described briefly. For the isolation of microsatellite loci in animals, an enrichment method was introduced. And then, some practical problems of this technique were summarized.