Detection of human cytomegalovirus infection by FQ-PCR technique and its application in the diagnosis and treatment of HCMV infected children
Chen, F.; He, Z.; Pan, S.; Ning, F.; Wang, Q.; Xiao, Z.
Zhonghua Shi Yan He Lin Chuang Bing du Xue Za Zhi 16(2): 187-190
2002
ISSN/ISBN: 1003-9279 PMID: 12196838 Document Number: 548102
Samples from 45 children suspected of having human cytomegalovirus (HCV) infection were examined using polymerase chain reaction (PCR), ELISA and fluorescent quantitative (FQ)-PCR. 25 HCV hepatitis cases were randomly assigned to receive ganciclovir combined with intravenous immunoglobulin (GCV + IVIG) or a control group. Patients from both groups were administered prednisone, glucurone, luminal and Xiaoyanlidanpian. The positive rates for PCR, ELISA and FQ-PCR were 60.0%, 33.33% and 66.67%, respectively, and their sensitivities were 84.38%, 46.88% and 93.75%, respectively. At baseline, there was no significant difference in viral DNA levels between the 2 treatment groups. Although viral load in both treatment groups decreased, the viral load in the group that received GCV + IVIG had more significant decreases (P<0.001). Results indicate that a viral level of 103 copies/ml detected using PQ-PCR can be taken as critical value for prediction of active HCV infection.