Protein and mRNA expression of nitric oxide synthase isoforms i and IIi in rat penile spongium tissues
Shi, J.-P.; Zhao, Y.-M.; Qi, G.
Zhonghua Nan Ke Xue 9(9): 663
2003
ISSN/ISBN: 1009-3591 PMID: 14727352 Document Number: 563854
To investigate protein and mRNA expression of nitric oxide synthase (NOS) isoforms I and III in rat penile tissues. Penile tissues from male SD rats were investigated. The protein expression of NOS I and III in rat penis was confirmed by immunoprecipitation and Western blot. Gene expression of both NOS I and III was identified by reverse transcription-polymerase chain reaction. The mRNA and protein products of NOS I and III are both expressed in normal rat penile spongium tissues. But, NOS III mRNA and protein products are more highly expressed than those of NOS. These findings support the possibility that penile erection is regulated by different NOS isoforms in rat penile tissues.