Immunohistochemical expression of steroid receptors and glycodelin A in isolated proliferative human endometrial glandular cells after stimulation with tamoxifen and phytoestrogens (genistein and daidzein)
Mylonas, I.; Jeschke, U.; Makovitzky, J.; Winkler, L.; Richter, D.U.; Friese, K.; Briese, V.
Anticancer Research 23(2a): 1119-1125
2003
ISSN/ISBN: 0250-7005 PMID: 12820358 Document Number: 563622
Aims: To evaluate the distribution patterns of steroid hormone receptors (oestrogen receptor (ER) and progesterone receptor (PR)) and glycodelin A (GdA) expression of proliferative endometrial glandular cells after stimulation with tamoxifen (TAM) and phytoestrogens (PE) genistein and daidzein. Materials and methods: Human endometrium was obtained from 4 premenopausal women. Glands were stimulated after isolation with single doses of TAM, genistein and daidzein (0.1, 1 and 10 micro mol/litre) and examined for ER, PR and GdA after 9 days of culture. Results: ER showed a significant decline with the highest TAM and genistein concentrations (P<0.05), whereas PR increased significantly with TAM and genistein concentrations of 1 and 10 micro mol/litre (P<0.05). GdA did not show any significant expression under TAM and genistein stimulation. Stimulation with daidzein resulted in no statistically relevant alterations in ER, whereas PR significantly increased with all 3 concentrations (P<0.05) and GdA also showed a significant increase with 1 micro mol/litre (P<0.05). Discussion: TAM showed anti-oestrogenic properties in premenopausal endometrium. PE showed similar ER and PR expression patterns as TAM, thus PE (genistein and daidzein) could also act as anti-oestrogens. GdA marked a cell transformation from proliferative to secretory status or the anti-oestrogen effects of TAM and PE.