Effect of Sinorhizobium fredii YC4 symbiotic plasmid amplification on nod factors and symbiotic N-fixation
Miao, L.; Zhou, J.
Ying Yong Sheng Tai Xue Bao 14(9): 1517-1520
2003
ISSN/ISBN: 1001-9332 PMID: 14733011 Document Number: 560238
Sinorhizobium fredii YC4 can form nitrogen-fixing nodules on soybean (Glycine max) and wild soybean (G. soja). It can produce unique lipochitooligosaccharide nod factors (LCOs), in comparison with the other four strains of S. fredii. The constitution of LCOs produced by YC4 contained more hydrophobic substitutions detected by Thin-layer chromatography (TLC) of 14C-labeled nod factors. A spontaneous mutant termed YSC3 amplified in the symbiotic plasmid was isolated from YC4, which can produce more amount of LCOs than its parental strain at 28 degrees C, and showed a difference in the construction of LCOs. Nodulation test indicated that YSC3 only formed ineffective nodules on soybean G. soja.