Laboratory culture of Tetraselmis strains isolated from the coasts of China
Ouyang, Y.; Luo, L.; Hu, H.; Zhang, C.; Li, Y.
Ying Yong Sheng Tai Xue Bao 14(10): 1701-1704
2003
ISSN/ISBN: 1001-9332 PMID: 14986369 Document Number: 559347
Tetraselmis is an important alga commonly used as live food in aquaculture. This paper reported the laboratory culture of its strains collected from the coasts of China, covering Guangdong, Zhejiang and Shandong Provinces. The separated strains of Tetraselmis for mono-species culture were isolated with capillary method, and their axenic cultures were obtained by inoculating them in plates with solid Marine III medium (M III). Modified Guillard & Ryther medium (MGM), M III, and modified Marine III medium (M III M) were utilized for the liquid cultivation of Tetraselmis in 500 mL flasks. It was displayed that the average growth of Tetraselmis cultured in M III M was the fastest at pH 7.0-8.0, and vitamin B12 addition to M III M slightly increased the relative growth rate of Tetraselmis. Therefore, the modified Marine III medium was effective to use as a large-scale culture medium for Tetraselmis in aquaculture.