In vivo model for dyslipidemia with diabetes mellitus in hamster
Bhatia, G.; Rizvi, F.; Saxena, R.; Puri, A.; Khanna, A.K.; Chander, R.; Wulff, E.M.; Rastogi, A.K.
Indian Journal of Experimental Biology 41(12): 1456-1459
2003
ISSN/ISBN: 0019-5189 PMID: 15320502 Document Number: 559207
Due to similarities in lipid metabolism to those in humans, hamster is considered as a good model for the study of regulatory mechanisms of plasma lipoproteins in response to cholesterol or fatty acid-enriched diet. This model of hyperlipidemia has been modified to produce dyslipidedmia with diabetes complexities by feeding with high fat diet added with 9% (w/w) fructose. Feeding this diet to hamster for 10 days markedly increases plasma levels of triglyceride, cholesterol, fatty acids followed by a significant increase in glycerol, beta lipoproteins, high density lipoprotein, glucose and glycosylated proteins. This model is being used for research and development of lipid lowering drugs with hypoglycemic activity in collaboration with Novo Nordisk, Denmark. The modified high fat diet formulation has now been prepared (Research diet D.99122211) and supplied by Research Diets Inc, Burnswick USA.