Expression of urotensin Ii mRNA in normal adrenal medulla and pheochromocytoma tissues
Liu, G.-q.; Zeng, Z.-p.; Li, H.-z.; Fan, X.-r.; Liu, D.-m.; Tong, A.-l.; Zheng, X.
Zhonghua Yi Xue Za Zhi 83(14): 1225-1228
2003
ISSN/ISBN: 0376-2491 PMID: 12930635 Document Number: 558952
To investigate the expression of human urotensin II (UII) mRNA in normal adrenal medulla and pheochromocytoma tissues. The total RNA of normal adrenal medulla from 9 males who died of accidents and the total RNA of pheochromocytoma tissues resected from 19 patients were extracted. The expression of UII mRNA was studied by reverse transcription-polymerase chain reaction (RT-PCR). UII and its internal control GAPDH were co-amplified in the same tube. Semi-quantitative analysis of the mRNA expression level of UII was carried out based on the ratios of the grey scale of the amplified target band to that of the control band (UII/GAPDH). The amplified product of UII is a 385 bp fragment and 99.7% of the bases were the same as the sequence registered in the GenBank. The UII/GAPDH in the normal adrenal medullae and pheochromocytomas were 0.69 +/- 0.29 (n = 9) and 0.22 +/- 0.18 (n = 19) respectively (P < 0.05). Both the normal adrenal medulla and pheochromocytoma express UII mRNA. The expression of UII mRNA is lower in pheochromocytoma than in normal adrenal medulla.