Studies on callus growth and phillyrin accumulation of Forsythia suspensa
Liu, Y-qi.; Tang, X-chun.; Cai, D-tian.
Zhongguo Zhong Yao Za Zhi 28(4): 321-323
2003
ISSN/ISBN: 1001-5302 PMID: 15139138 Document Number: 558586
To investigate the effects of physical and chemical factors on callus growth and phillyrin contents of F. suspensa. The cell growth index and phyllirin yield in different culture condition such as different plant hormones mixed, mediums, light and dark were compared. HPLC was used to examine phillyrin contents. Growth cycle of cells is twenty-eight days. During the course of callus growth, the processes of phillyrin biosynthesis were parallel with the cell growth. The optimum medium is MS. The optimum hormones concentrations are 1 mg.L-1 2,4-D, 0.5 mg.L-1 6-BA and 0.5 mg.L-1KT. The cell culture in light is more suitable than that in dark.