Laminin and its alpha 6 integrin receptor in the regulation of human hepatocellular carcinoma cell phenotypes
Lu, T-jing.; Zhang, Q-yun.; Zhou, R-li.
Zhonghua Zhong Liu Za Zhi 25(1): 31-35
2003
ISSN/ISBN: 0253-3766 PMID: 12678983 Document Number: 558400
Objective To study the role of integrin alpha6 in cell attachment, spread, survival/proliferation and differentiation of human hepatocellular carcinoma (HCC) BEL-7402 cells on various substrates by monoclonal antibody against the extracellular domain of alpha6 subunit (IA6ED McAb). Methods The effect of McAb on attachment and spread of BEL-7402 cells on LN or FN substrate was examined. MTT analysis was used to examine the cell survival/proliferation, gelatin zymography to the matrix metalloproteinases (MMPs) secreted by BEL-7402 cells, and micropartical immunoabsorbant kit to AFP secretion while the cells were cultured on LN-, FN- or Matrigel-coated substrates. Results The cell attachment, spread and survival/proliferation were inhibited. Moreover importantly, the malignant cell dedifferentiation and abnormal differentiation on LN-coated substrate were also strongly inhibited by IA6ED McAb. Conclusion LN and integrin alpha6 regulate human HCC cell phenotypes of survival/proliferation and differentiation. The phenotypes of dedifferentiation and abnormal differentiation can be reversed by blocking the interaction between integrin alpha6 and LN using IA6ED McAb, which may lower metastatic potency of tumor cells.