Standardization of glycosylated hemoglobin in the context of the DAI study
Mosca, A.; Paleari, R.
Annali Dell'istituto Superiore di Sanita 39(2): 145-151
2003
ISSN/ISBN: 0021-2571 PMID: 14587212 Document Number: 558028
The results obtained from 85 antidiabetic centers enrolled in the DAI study are presented with regard to the external quality assessment scheme for glycohemoglobin. The materials have been prepared by a laboratory of the network of reference laboratories of the International Federation of Clinical Chemistry (IFCC). To each control a Diabetes Control and Complications Trial (DCCT) traceable target value was assigned. The High-Performance Liquid Chromatography (HPLC) methods for glycohemoglobin are used in 75% of the centers, the immunochemical techniques in 21% and less than 5% is using affinity chromatography based methods. The data collected from the laboratories who completed the set of measurements show that 64% of the centers are well aligned to the DCCT system. The reproducibility of the methods varied between 3.7 and 5.8% (as CV, %) and has to be improved.