Production and characterization of a panel of monoclonal antibodies to Toxoplasma gondii antigens
Poryvaeva, V.A.; Aborneva, I.V.; Bormotov, N.I.; Gladkova, S.E.; Reshetnikov, S.S.; Kuvshinova, I.N.; Rukavishnikov, M.I.; Dedkova, L.M.; Belanov, E.F.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 4: 20-24
2003
ISSN/ISBN: 0208-0613 PMID: 14664158 Document Number: 557654
A representative collection was obtained containing 68 monoclonal antibodies (MAB) to Toxoplasma gondii antigens, which was characterized by the binding with the below fractions of tochizoites in the immune-enzyme assay (IEA) and immunoblotting (IB): membrane (MEM), somatic (water-soluble, SOM) and excretory-secretory (ES). Most of MABs were produced to MEM antigens (43), 6 MABs reacted with the somatic fraction, and 3 MABs reacted with both fractions. Two MABs to ES antigen were detected in the latter group. An analysis of MABs in concurrent IEA and IB revealed the immune-dominant proteins of the MEM and SOM fractions of antibodies to T. gondii tochizoites (p30 and p27, respectively). The presence of 2 non-overlapping antigenic determinants was shown for p30. Further research would detect MABs that could be used in the diagnosis of toxoplasmosis.