Molecular responses of rice (Oryza sativa L.) to the stress of lowly enhanced UV-B radiation
Zeng, J.; Lin, W.; Liang, K.; Chen, F.; Guo, Y.; Tang, L.; He, H.; Wang, J.; Liang, Y.
Ying Yong Sheng Tai Xue Bao 14(6): 941-944
2003
ISSN/ISBN: 1001-9332 PMID: 12974001 Document Number: 553515
The approach of mRNA differential display (DD) was used to analyze the gene differential expression of rice seedlings subjected to lowly enhanced Ultraviolet-B radiation. The results showed that of the eighteen differential fragments, fifteen ones were different in expressional levels, another one was depressed, and the other two, named as RUVB1 and RUVB2 respectively, which could be recovered and re-amplified, were specifically induced. Sequence analysis for RUVB2 through Internet BLAST searching indicated that it was more than 95% identities with three rice ESTs, but very low similarity to others in the GenBank, and was mapped on the OS-JNBb0091N21BAC(GenBank accession No.: AC091122) in Oryza sativa L.