Search for non-relative donor by the Russian register of bone marrow donors
Zaretskaia, I.M.; Khamaganova, E.G.; Aleshchenko, S.M.; Murashova, L.A.
Terapevticheskii Arkhiv 74(7): 30-35
2002
ISSN/ISBN: 0040-3660 PMID: 12181831 Document Number: 549883
Aim: To select maximally HLA compatible donor for hematological patients who need transplantation of bone marrow from non-relative donor. Materials and methods: 75 patients with hematological malignancy were observed. All of them have indications to non-relative transplantation of the bone marrow. Methods of polymerase chain reaction with sequence-specific primers and classic microlymphocytotoxic test were used. Results: Typing of HLA antigens of class I and alleles of class II loci enabled search for non-relative donor for transplantation of bone marrow in accordance with the requirements of the European Federation of Immunogenetics. Most of the patients (86,6%) had at least one potential HLA-A,-B,-DR compatible donor. Half of the patients had potential donors typed at the allele level by class II loci. This diminishes time of HLA compatible donor selection. Conclusion: DNA typing enables the search for the non-relative donors meeting modern requirements. This allowed 5 non-relative bone marrow transplantations.