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Preliminary study on hens immunized with recombinant human testis prostaglandin D synthase DNA

Lu, J.-C.; Huang, Y.-F.; Zhang, X.-R.

Zhonghua Nan Ke Xue 8(1): 22-24

2002

ISSN/ISBN: 1009-3591
PMID: 12479041
Document Number: 548958
In order to identify whether the hens immunized with recombinant human testis prostaglandin D synthase (rhtL-PGDS) DNA can produce anti-L-PGDS antibody. The serum were got from the hens immunized with recombinant plasmid pGEX-2T/htL-PGDS DNA (100 micrograms) every 2 weeks for 2 times. The exist of anti-L-PGDS antibody and its titer were tested with agarose dual immunodiffusion and ELISA with rhtL-PGDS as antigen. The serum anti-L-PGDS antibody in hen immunized with pGEX-2T/htL-PGDS DNA were confirmed and its titer tested by ELISA was 1:2,048. It is feasible to produce anti-L-PGDS antibody by immunizing hens with recombinant pGEX-2T/htL-PGES DNA.

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