A sieving method for collecting the metacercariae of trematode parasites from freshwater fish
Fan, P.C.; Wu, C.C.; Huang, P.; Yen, C.W.
Southeast Asian Journal of Tropical Medicine and Public Health 33(1): 23-24
2002
ISSN/ISBN: 0125-1562 PMID: 12118453 Document Number: 544093
This study describes a sieving method for the collection of metacercariae from frozen (-20 degrees C) freshwater fish. Digested fish tissue is filtered through a series of sieves; the crude filtrate is then centrifuged. Centrifugation produces a sediment from which metacercariae can be removed. Half of the metracercariae that were obtained from the fish meat that had been frozen for 10 days (-20 degrees C) were dead; the other half were alive and some larvae were moving slowly.