The coexpression of the preS1 (1-42) and the core (1-144) antigen of HBV in E. coli
Zhao, Y.; Zhan, M.
Chinese Medical Sciences Journal 17(2): 68-72
2002
ISSN/ISBN: 1001-9294 PMID: 12906156 Document Number: 543639
To study the therapeutic T cell vaccine for the treatment of chronic hepatitis B by improving the cellular immunization of HBsAg vaccine with the coexpression of the preS1 (1-42) and the Core (1-144) antigen of HBV in E. coli. The genes of HBcAg (1-144) and preS1 (1-42) were amplified and fused by PCR. This fused gene was inserted in the prokaryotic expression vector pET-11d and expressed in E. coli. It was showed by SDS-PAGE that the protein molecular weight of the coexpression product was about 20 kD, 20% of all bacteria protein. The monoclonal antibodies against core and preS1 antibody could react with this fused protein by Western-blot technique respectively. The fused gene was verified by sequencing. Under the immune electron microscopy, this fused protein is typical particle of HBcAg but in an aggregated form. The results might aid for studying T cell immunotherapeutic vaccine for chronic hepatitis B.