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Use of the hepatitis B core antigen produced in Escherichia coli in an assay for anti-HBc

Peutherer, J.F.; MacKay, P.; Ross, R.; Stahl, S.; Murray, K.

Medical Laboratory Sciences 38(4): 355-358

1981

ISSN/ISBN: 0308-3616
PMID: 7035782
Document Number: 179332
Extracts of cultures of E. coli carrying a plasmid with hepatitis B viral DNA contain a polypeptide that reacts with antibody to hepatitis B core antigen (anti-HBc). The antigen can be used in a radioimmunoassay for anti-HBc, either in microtiter plates coated with anti-HBc or by coating beads. The E. coli HBc antigen, liver antigen and a commercial assay gave very similar results in tests of 264 sera. The assay was also used to test 593 sera from patients attending a clinic for sexually-transmitted diseases.

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