Galactomannan from ambiguous Crazyweed (Oxytropis ambigua (Pall) DC)
Mestechkina, N.M.; Lobanova, I.E.; Anulov, O.V.; Shcherbukhin, V.D.
Prikladnaia Biokhimiia i Mikrobiologiia 37(4): 453-456
2001
ISSN/ISBN: 0555-1099 PMID: 11530670 Document Number: 539943
A galactomannan with a molecular weight of 735 kDa was first isolated and purified from seeds of ambiguous crazyweed Oxytropis ambigua (Pall) DC. (family Leguminosae) with a yield of 3.6%. Its aqueous solutions displayed an optical activity (ɑD = 73.32 degrees) and high viscosity ( = 644 ml g-1). Chemical analysis and 13C-NMR spectroscopy revealed the presence in the heteropolysaccharide of D-mannopyranose and D-glucopyranose at a molar ratio of 1.39:1. The linear backbone of its macromolecule consists of 1.4-beta-D-mannopyranose residues. Single beta-D-galactose residues substitute 72% of mannoses to form branches.