Effects of musk glucoprotein on the function of rat polymorphonuclear leukocytes activated by IL-8 in vitro
Wang, W.J.; Bai, J.Y.; Cheng, G.F.; Zhu, X.Y.
Zhongguo Zhong Yao Za Zhi 26(1): 50-53
2001
ISSN/ISBN: 1001-5302 PMID: 12525122 Document Number: 538915
To investigate the effects of musk-1, a glucoprotein component isolated from the water extract of musk, on some functions of rat polymorphonuclear leukocytes activated by IL-8 in vitro. An in vitro incubation system was used. Superoxide anion production was determined by cytochrome C reduction. beta-glucuronidase and lysozyme release was quantitated by enzyme reactions in which phenolph-thaleinglucuronic acid and Micrococcus Lysodeikticus were as the substrates, respectively. In comparison with control, musk-1 at concentration 1-100 micrograms.ml-1 can increase superoxide anion production by 91.7%-291%, and decrease beta-glucuronidase and lysozyme release by 2.2%-58.1% and 3.9%-39.8%, respectively. Inhibition of lysosomel enzyme release might be considered as one of mechanisms of antiinflammatory action of musk.