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Astrovirus detection in wastewater samples

Pintó, R.M.; Villena, C.; Le Guyader, F.; Guix, S.; Caballero, S.; Pommepuy, M.; Bosch, A.

Water Science and Technology a Journal of the International Association on Water Pollution Research 43(12): 73-76

2001

ISSN/ISBN: 0273-1223
PMID: 11464772
Document Number: 538074
Procedures for the detection of astroviruses in wastewater samples have been developed and evaluated. Following these methodologies, we investigated the occurrence of astroviruses in wastewater samples from three different sewage treatments plants located in Southern France and two in the Barcelona area. Some positive samples were genotyped by analysis of a fragment of the ORF1a by restriction fragment length polymorphism (RFLP) with endonuclease DdeI. The amplimers generated contain several sites for the DdeI restriction endonuclease, being the number and location of sites different between strains.

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