Formation of xylitol in Candida guilliermondii 2581 culture
Zagustina, N.A.; Rodionova, N.A.; Mestechkina, N.M.; Shcherbukhin, V.D.; Bezborodov, A.M.
Prikladnaia Biokhimiia i Mikrobiologiia 37(5): 573-577
2001
ISSN/ISBN: 0555-1099 PMID: 11605470 Document Number: 529497
The yeast strain Candida guilliermondii 2581 was chosen for its ability to produce xylitol in media with high concentrations of xylose. The rate of xylitol production at a xylose concentration of 150 g/l is 1.25 g/l per h; the concentration of xylitol after three days of cultivation is 90 g/l; and the relative xylitol yield is 0.6 g per g substrate consumed. The growth conditions were found that resulted in the maximum relative xylitol yield with complete consumption of the sugar: xylose concentration, 150 g/l; pH 6.0; and shaking at 60 rpm. It was shown that the growth under conditions of limited aeration favors the reduction of xylose.